L. and R. Hand might be based off of Mario and SMG4.
This is SMG4's first 2014 video.
In the actual game, these hands are called Eyerok, but in the blooper, SMG4 just randomly calls them L. and R. Hand.
This is the first and only video where Eyerok has a major appearance.
The hands own a handmobile; the last time they drived the handmobile was 4,000 years ago, but obviously, SMG4 is making a joke because cars didn't exist at that time.
The title is a reference to the word "handjob" as well as the video 2 Girls 1 Cup.
It is said that Bowser went to get a pregnancy test. This could possibly be a nod to the ending sequence of The Mario Parable, when the narrator says that a doctor reported that Bowser was pregnant; to add to the joke SMG3 (who was there when Bowser mentioned this) says the exact same thing Bowser said upon hearing this (the exact same voice clip was used).
Ragnorok is also the name of the norse God of The Apocalypse, which the wolf was symbolized. The naming of the character might be to make contrast, as Ragnorok is "gay pink".
This blooper is the start of Season 4 in SMG4's bloopers.