The Old Lady is an elderly character based on the Matron trainer class from Pokémon, which first appeared in Pokémon Ruby and Sapphire. While not inherently aggressive, the Old Lady is often angered by Mario's and others' antics.
The Old Lady's personality is very similar to that of the Old Man Hobo only a bit more aggressive or otherwise mildly angered at times.
- She appeared in a lot of videos from 2014 to 2016 although she disappeared entirely in 2017.
- It is unknown if she will appear again.